Microfluorometric assay for quantification of anti-erythrocytic antibody level in sera of Plasmodium berghei ANKA infected semi-immune mice

A simple, sensitive and reproducible fluorometry method was developed to quantify anti-erythrocytic auto-antibody in the sera of semi-immune mice. The level of anti-erythrocytic auto-antibody measured using fluorometry was significantly higher in sera of Plasmodium berghei ANKA infected mice than the uninfected, p< 0.0001. This result correlates significantly with the standard ELISA assay, p<0.0001, r=0.7766. Both methods show significant correlation with regard to extent of Hb loss, p<0.006 (r=0.33) (fluorometry method) and p< 0.0001, r=0.30 (ELISA). Thus the fluorometry method may serve as an alternative to standard ELISA assay and can be modified to suit similar objectives.